Info-enriched characterization of cell populations. Morphological data from pictures can increase to the richness of flow cytometry facts. One example is, the determine shows an otherwise typical apoptosis assay using Annexin V and PI, incorporating mobile imaging to characterize cells in Every single inhabitants to expose morphologically distinctive capabilities. These insights could not are already attained from multiplex staining by yourself.
Whether you’re a newbie or an expert, check out our means to progress your investigation and know-how in flow cytometry.
Merge acoustic concentrating know-how with large-parameter analysis for flexible spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a superior-pace brightfield digital camera for simultaneous large throughput flow cytometry and substantial resolution brightfield imaging.
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In this article, a highly skilled person has gated singlets confidently. Right after evaluating the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of much more than 4% aggregates.
Optimizing gating strategy. Even robust manual singlet gating is error-prone and remains a subjective determination place in Pretty much all flow cytometry assays. Imaging can be used to substantiate and alter gates to include only solitary cells of fascination.
Our scientists will structure panels for you as over and over you need—free of charge, no product or service obtain necessary.
The Attune NxT cytometer will be the dependable workhorse instrument your lab can depend on for dependable and reliable overall performance. The Attune CytPix model contains a substantial-speed brightfield camera and AI-pushed image Evaluation program, with new upgrades including device learning applications and an intuitive person interface, enabling enhanced data precision, precision, and more quickly insights into sample biology.
Buyers can check out celebration images in actual-time to confirm sample quality, improve protocols, and history the morphology of each cell populace discovered for analysis. Imaging capture also benefits from acoustic concentrating, which helps you to place the cells in order that a pointy, centered picture is received.
Sound waves help exact cell alignment, leading to Extraordinary facts high quality and ~10X more rapidly sample flow premiums.
We Formerly demonstrated the strength of imaging Auto-T/Ramos cell interactions. Allow’s evaluate just the populace of best curiosity, the double constructive occasions, To find out more. We can now use extended morphological parameters (in this article, circularity and skewness of intensity) to further more analyze the options of such populations and refine gating on these occasions, increasing facts robustness.
Leverage the power of State-of-the-art technological know-how and innovation to speed up your discoveries. Rely on within the Attune Flow Cytometer spouse and children to deliver the dependability, speed, and accuracy that set your get the job done aside.
Flexible payment strategy from Agilent Financial Methods let you obtain the crucial instrumentation you'll need for rapid challenge, or to improve your lab.
Gating on these prolonged parameters lets you rapidly and precisely establish populations of curiosity and ensure gating approach with little or no guide overview. The Attune software gives access to state-of-the-art machine Finding out Investigation equipment inside an expertly built consumer interface, making it extremely easy to use.
As the cell line experienced Beforehand been analyzed around the Attune CytPix Flow Cytometer, the scientists went back again to the photographs and were capable of doc the microbial infection at least 5 days before.
The Attune https://collintlvdm.pages10.com/flow-cytometer-a-revolutionary-tool-for-cell-analysis-69073010 CytPix Flow Cytometer enables buyers to visualize samples through acquisition, verify the accuracy of gating techniques, and aid effects with dependable documentation for publication applications.
In this article, we present that by gating strategies working with graphic-based quantitative parameters can distinguish interacting cells from coincident situations to much more properly evaluate interacting cells.